Unit 1: Introduction to Laboratory Techniques, Biosafety, and Laboratory Safety

• Overview of microbiology and molecular biology
• Significance of laboratory techniques in research and diagnostics
• Fundamentals of laboratory safety and biosafety
• Laboratory dos and don’ts
• Identifying laboratory hazards
• Personal protective equipment (PPE) and its role
• Emergency procedures and waste disposal protocols

Unit 2: Media Preparation and Sterilization

• Overview of media types used in microbiology
• Preparation techniques for agar and broth media
• Methods for sterilizing media
• Autoclave operation and maintenance
• Decontamination processes
• Glassware preparation techniques

Unit 3: Sterile Techniques and Culture Methods

• Sterilization methods using hot air ovens
• Types and usage of biosafety cabinets
• Importance of sterile techniques in microbiological practices
• Techniques for culturing bacteria and other microorganisms
• Hands-on practical sessions: inoculation and incubation

Unit 4: Centrifugation and Pipetting Techniques

• Principles and applications of centrifugation
• Hands-on training in centrifugation practices
• Accurate pipetting techniques and best practices

Unit 5: Molecular Biology Techniques

• Isolation of bacteria and bacterial DNA
• Hands-on practical session focusing on PCR (Polymerase Chain Reaction)

Unit 6: Identification of Bacteria

• Polyphasic approaches for bacterial identification
• Techniques for characterizing bacteria
• Understanding bacterial growth curves

Unit 7: Critical Thinking and Problem-Solving in the Laboratory

• Engaging in experimental design and troubleshooting methodologies
• Case studies and group discussions to foster collaborative learning
• Problem-solving exercises focused on laboratory techniques

1. Understanding Techniques: Grasp and apply various laboratory techniques utilized in microbiology and molecular biology.
2. Proficiency in Instrumentation: Achieve proficiency in operating laboratory instruments and equipment.
3. Practical Skill Development: Cultivate practical skills in sterile techniques, culture methods, and molecular assays.
4. Safety Awareness: Foster awareness of laboratory safety and biosafety practices.
5. Critical Thinking Enhancement: Improve critical thinking and problem-solving skills through engaging hands-on experiments.